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GHS Classification Result

GENERAL INFORMATION

Item	Information
CAS RN	74115-24-5
Chemical Name	3,6-Bis(2-chlorophenyl)-1,2,4,5-tetrazine
Substance ID	25B0049
Classification year (FY)	FY2013
Ministry who conducted the classification	Ministry of Health, Labour and Welfare (MHLW)/Ministry of the Environment (MOE)
New/Revised	Revised
Classification result in other fiscal year	FY2006
Download of Excel format	Excel file

REFERENCE INFORMATION

Item	Information
Guidance used for the classification (External link)	GHS Classification Guidance by the Japanese Government (July, 2013)
UN GHS document (External link)	UN GHS document
Definitions/Abbreviations (Excel file)	Definitions/Abbreviations
Model Label by MHLW (External link)	MHLW Website (in Japanese Only)
Model SDS by MHLW (External link)	MHLW Website (in Japanese Only)
OECD/eChemPortal (External link)	eChemPortal

PHYSICAL HAZARDS

Hazard class		Classification	Pictogram Signal word	Hazard statement (code)	Precautionary statement (code)	Rationale for the classification
1	Explosives	Classification not possible	- -	-	-	There is a chemical group associated with explosive properties (neighboring nitrogen atoms) in the molecule, but the classification is not possible due to no data. Besides, it is used as a pesticide (miticide), not as explosives.
2	Flammable gases (including chemically unstable gases)	Not applicable	- -	-	-	Solid (GHS definition)
3	Aerosols	Not applicable	- -	-	-	Not aerosol products.
4	Oxidizing gases	Not applicable	- -	-	-	Solid (GHS definition)
5	Gases under pressure	Not applicable	- -	-	-	Solid (GHS definition)
6	Flammable liquids	Not applicable	- -	-	-	Solid (GHS definition)
7	Flammable solid	Classification not possible	- -	-	-	No data available.
8	Self-reactive substances and mixtures	Classification not possible	- -	-	-	There is a chemical group associated with explosive properties (neighboring nitrogen atoms) in the molecule, but the classification is not possible due to no data.
9	Pyrophoric liquids	Not applicable	- -	-	-	Solid (GHS definition)
10	Pyrophoric solids	Classification not possible	- -	-	-	No data available.
11	Self-heating substances and mixtures	Classification not possible	- -	-	-	No data available.
12	Substances and mixtures which, in contact with water, emit flammable gases	Not applicable	- -	-	-	The chemical structure of the substance does not contain metals or metalloids (B, Si, P, Ge, As, Se, Sn, Sb, Te, Bi, Po, At).
13	Oxidizing liquids	Not applicable	- -	-	-	Solid (GHS definition)
14	Oxidizing solids	Not applicable	- -	-	-	The substance is an organic compound containing chlorine (but not fluorine or oxygen) which is chemically bonded only to carbon or hydrogen.
15	Organic peroxides	Not applicable	- -	-	-	Organic compounds containing no bivalent -O-O- structure in the molecule.
16	Corrosive to metals	Classification not possible	- -	-	-	Test methods applicable to solid substances are not available.

HEALTH HAZARDS

Hazard class		Classification	Pictogram Signal word	Hazard statement (code)	Precautionary statement (code)	Rationale for the classification
1	Acute toxicity (Oral)	Not classified	- -	-	-	It was classified as "Not classified" based on an LD50 value of > 3,200 mg/kg for rats (RTECS (2013), the original article: British Crop., Protection Council (1981)). Besides, the classification was conducted based on data in RTECS (2013) obtained in this investigation.
1	Acute toxicity (Dermal)	Classification not possible	- -	-	-	Classification not possible due to lack of data.
1	Acute toxicity (Inhalation: Gases)	Not applicable	- -	-	-	Solid (GHS definition)
1	Acute toxicity (Inhalation: Vapours)	Not applicable	- -	-	-	Solid (GHS definition)
1	Acute toxicity (Inhalation: Dusts and mists)	Classification not possible	- -	-	-	Classification not possible due to lack of data.
2	Skin corrosion/irritation	Not classified	- -	-	-	In a skin irritation/corrosion test with six guinea pigs, as a result of the 24-hour application of 66.6 mg/0.2 mL of this substance to the skin, very slight edema was observed on 2/12 sites. No systemic toxicity was seen at necropsy after the 7-day observation period, and no histopathological changes were found in the samples of the skin where this substance was applied (JMPR (2005)). It is concluded in JMPR (2005) that this substance caused negligible irritation to the skin of guinea pigs. It was classified as "Not classified" in the Classification JIS (Category 3 in UN GHS classification) based on the above information. The information in JMPR (2005) obtained in this investigation was added. "Not classified" in the Classification JIS (Category 3 in UN GHS classification) was specified based on the GHS classification guidance for the Japanese government.
3	Serious eye damage/eye irritation	Category 2B	- Warning	H320	P305+P351+P338 P337+P313 P264	In an eye irritation/corrosion test with six rabbits (according to OECD TG 405), mild conjunctival hyperemia was observed in 6/6 animals, but this returned to normal within two days after application. No discharge, chemosis, corneal damage, or iridial inflammation was seen (JMPR (2005)). It was classified in Category 2B based on the above information. The information in JMPR (2005) obtained in this investigation was added.
4	Respiratory sensitization	Classification not possible	- -	-	-	Classification not possible due to lack of data.
4	Skin sensitization	Not classified	- -	-	-	In JMPR (2005) obtained in this investigation, it is reported that in a maximization test with 20 guinea pigs, mild redness was observed in 1/20 animals 24 hours after the removal of the challenge patch, but this disappeared 48 hours after the removal of the patch, and it is concluded in JMPR (2005) that it was not sensitizing to the skin of guinea pigs. Therefore, it was classified as "Not classified." The information in JMPR (2005) obtained in this investigation was added.
5	Germ cell mutagenicity	Classification not possible	- -	-	-	It was classified as "Classification not possible" because it was not possible to classify a substance as "Not classified" according to the revised GHS classification guidance for the Japanese government. As for in vivo, it was negative in a dominant lethal test with rats and a micronucleus test with mouse bone marrow cells (JMPR (2005), IRIS (2012)). As for in vitro, it was negative in a bacterial reverse mutation test, and a gene mutation test and a chromosomal aberration test with cultured mammalian cells (JMPR (2005), IRIS (1991)).
6	Carcinogenicity	Category 2	Warning	H351	P308+P313 P201 P202 P280 P405 P501	It was classified in Category 2 because the EPA classified it in C (IRIS (1991)). The category was changed according to the revised GHS classification guidance for the Japanese government.
7	Reproductive toxicity	Not classified	- -	-	-	It was classified as "Not classified" because no reproductive toxicity was seen in a multi-generation reproductive toxicity test with rats, and no developmental toxicity was observed even at the doses where maternal toxicity was found in developmental toxicity tests with rats and rabbits (JMPR (2005)). The category was changed because information on reproductive/developmental toxicity was obtained.
8	Specific target organ toxicity - Single exposure	Classification not possible	- -	-	-	Classification not possible due to lack of data. Besides, it corresponds to "Not classified" in the oral route because no toxic signs were observed at the doses within the guidance value range in rats, mice, and hamsters (JMPR (2005)).
9	Specific target organ toxicity - Repeated exposure	Category 2 (liver)	Warning	H373	P260 P314 P501	The liver was considered to be the target organ because it is described that centrilobular hypertrophy and vacuolation of hepatocytes, focal cystic degeneration of hepatocytes, and diffuse distribution of fat deposits in the liver were observed in a 2-year diet administration test with rats (IRIS (1991)), and eosinophilia of the periportal hepatocytes was found in a 12-month diet administration test with dogs (JMPR (2005)). Besides, effects in experimental animals were seen within the guidance value range (20-36 mg/kg/day) corresponding to Category 2. From the above, it was classified in Category 2 (liver). Besides, the blood system was included in the target organ in the previous classification because a significant decrease in methemoglobin was seen, however, hemoglobin was mistaken for methemoglobin, the decrease was described to be slight, and there were no related findings. Therefore, the blood system was removed from the target organ this time.
10	Aspiration hazard	Classification not possible	- -	-	-	Classification not possible due to lack of data.

ENVIRONMENTAL HAZARDS

Hazard class		Classification	Pictogram Signal word	Hazard statement (code)	Precautionary statement (code)	Rationale for the classification
11	Hazardous to the aquatic environment (Acute)	Not classified	- -	-	-	It was classified as "Not classified" from 96-hour LC50 > 80 microg ai/L for crustace, and 96-hour LC50 > 14.6 microg ai/L for fish (US EPA: RED, 2007). (ai: active ingredient)
11	Hazardous to the aquatic environment (Long-term)	Category 1	Warning	H410	P273 P391 P501	It was classified in Category 1 due to being not rapidly degradable (BIOWIN), and NOAEC = 6 microg ai/L in an ELS test for fish (US EPA: RED, 2007).
12	Hazardous to the ozone layer	Classification not possible	- -	-	-	This substance is not listed in the Annexes to the Montreal Protocol.

NOTE:

GHS Classification Result by the Japanese Government is intended to provide a reference for preparing a GHS label or SDS for users. To include the same classification result in a label or SDS for Japan is NOT mandatory.
Users can cite or copy this classification result when preparing a GHS label or SDS. Please be aware, however, that the responsibility for a label or SDS prepared by citing or copying this classification result lies with users.
This GHS classification was conducted based on the information sources and the guidance for classification and judgement which are described in the GHS Classification Guidance for the Japanese Government etc. Using other literature, test results etc. as evidence and including different content from this classification result in a label or SDS are allowed.
Hazard statement and precautionary statement will show by hovering the mouse cursor over a code in the column of "Hazard statement" and "Precautionary statement," respectively. In the excel file, both the codes and statements are provided.
A blank or "-" in the column of "Classification" denotes that a classification for the hazard class was not conducted in the year.

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