GHS Classification Result (Revision of the past classification result)

Chemical Name:Benzene, 1-methoxy-2-nitro-
CAS:91-23-6

Result:
ID: 22B4503
Classifier: Ministry of Health, Labour and Welfare (MHLW), Ministry of the Environment (MOE)
Year Classified: FY2010
Reference Manual: GHS Classification Guidance by the Japanese Government (July, 2010)

PHYSICAL HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
1 Explosives Not classified - - - - Although it contains chemical groups (nitro groups) associated with explosive properties in the molecule, it is UN2730 (nitroanisole (liquid)) Division 6.1 in United Nations Recommendations on the Transport and it is not classified into Class 1 Explosives.
2 Flammable gases (including chemically unstable gases) Not applicable - - - - Liquid (GHS definition)
3 Aerosols Not applicable - - - - Not aerosol products.
4 Oxidizing gases Not applicable - - - - Liquid (GHS definition)
5 Gases under pressure Not applicable - - - - Liquid (GHS definition)
6 Flammable liquids Not classified - - - - Its flash point 124 degC (measurement methods unknown) (ICSC (2004)) is over 93 degC.
7 Flammable solids Not applicable - - - - Liquid (GHS definition)
8 Self-reactive substances and mixtures Type G - - - - Although it contains chemical groups (nitro groups) associated with explosive properties in the molecule, it is UN2730 (nitroanisole (liquid)) Division 6.1, PG III in UNRTDG, therefore it was judged that it is classified into Type G.
9 Pyrophoric liquids Not classified - - - - Since its autoignition temperature is 464 degC (ICSC (2004)), it is considered that the substance does not ignite at room temperature, therefore the substance is classified as "Not classified".
10 Pyrophoric solids Not applicable - - - - Liquid (GHS definition)
11 Self-heating substances and mixtures Classification not possible - - - - Test methods applicable to liquid substances are not available.
12 Substances and mixtures which, in contact with water, emit flammable gases Not applicable - - - - The chemical structure of the substance does not contain metals or metalloids (B, Si, P, Ge, As, Se, Sn, Sb, Te, Bi, Po, At).
13 Oxidizing liquids Classification not possible - - - - Classification is not possible since no data are available though the substance is an organic compound containing oxygen which is bonded to nitrogen.
14 Oxidizing solids Not applicable - - - - Liquid (GHS definition)
15 Organic peroxides Not applicable - - - - Organic compounds containing no bivalent -O-O- structure
16 Corrosive to metals Classification not possible - - - - No data available.

HEALTH HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
1 Acute toxicity (Oral) Category 4 Warning H302: Harmful if swallowed P301+P312: IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell.
P264: Wash ... thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P330: Rinse mouth.
P501: Dispose of contents/container to ...
Five LD50 values for rats of 874, 740 (male) and 760 (female), 1000 (male) and 890 (female), 1980 mg/kg (DFGMAK-Doc. 9 (1998)) and 740 mg/kg (IARC 65 (1996)) were reported. Since all of the data corresponded to Category 4, the substance was classified as Category 4.
1 Acute toxicity (Dermal) Not classified - - - - Based on the rat LD50 value of > 2000 mg/kg (DFGMAK-Doc. 9 (1998)), the substance was classified as "Not classified". As relevant information, no mortality of the animal was observed at 2000 mg/kg.
1 Acute toxicity (Inhalation: Gases) Not applicable - - - - Liquid (GHS definition)
1 Acute toxicity (Inhalation: Vapours) Classification not possible - - - - No data available.
1 Acute toxicity (Inhalation: Dusts and mists) Classification not possible - - - - No data available.
2 Skin corrosion/irritation Not classified - - - - The substance was classified as "Not classified" based on the document that 4-hour application of 0.5 mL of undiluted substance did not cause any irritation to rabbit skin (DFGMAK-Doc. 9 (1998)).
3 Serious eye damage/eye irritation Not classified - - - - In the rabbit test, instillation of 0.1 mL of the substance to conjunctival sac caused swelling and reddening of conjunctiva after 1 hour, but all signs of irritation disappeared after 24 hours (DFGMAK-Doc. 9 (1998)). In another rabbit test, the substance caused no irritation (BUA 140 (1993)). Based on these documents, the substance was classified as "Not classified"
4 Respiratory sensitization Classification not possible - - - - No data available.
4 Skin sensitization Classification not possible - - - - No data available.
5 Germ cell mutagenicity Classification not possible - - - - The classification was not possible due to lack of in vivo test data. As relevant information, as for in vitro studies, positive results in multiple data of Ames test as well as chromosome aberration test using CHO cells or CHL cells were reported (DFGMAK-Doc. 9 (1998), Mutagenicity Test Data of Existing Chemical Substances based on the toxicity investigation system of the Industrial Safety and Health Law (1996)).
6 Carcinogenicity Category 2 Warning H351: Suspected of causing cancer P308+P313: IF exposed or concerned: Get medical advice/attention.
P201: Obtain special instructions before use.
P202: Do not handle until all safety precautions have been read and understood.
P281: Use personal protective equipment as required.
P405: Store locked up.
P501: Dispose of contents/container to ...
The substance was classified as Category 2 based on the carcinogenicity criteria of "Group 2B" in IARC (IARC 65 (1996)). As relevant information, in the 2-year dietary administration studies in rats and mice, the increases in the incidences of tumors in the urinary bladder and transitional epithelium of the kidney, large intestine, and mononuclear cell leukemia in rats, and increases in the incidences of tumors of hepatocytes in mice, were reported (NTP TR 416 (1993)).
7 Reproductive toxicity Classification not possible - - - - In the developmental toxicity study by oral administration during the organogenetic period in rats, increase in resorption, slight increase in visceral variations and fusions were observed at the high dose that clinical symptoms and reduction in food consumption were seen in the dams. But, teratogenic effects did not occur (DFGMAK-Doc. 9 (1998)). However, this study used a small number of animals and increase in resorptions were minor with 8.5% of its incidence in comparison with 5.0% in the control group. Therefore, these findings were not adopted to the basis for classification. In the 13-week oral administration study in rats, the effects on reproductive organs in females were reported ((DFGOT vol.9 (1998)). However, atrophic changes of reproductive organs in rats observed at the high dose level as 18000 ppm were considered to be non-specific secondary changes associated with weight loss. On the other hand, since there was no data on the sexual functions and fertility of parental animals, the substance was classified as "Classification not possible".
8 Specific target organ toxicity - Single exposure Category 2 (systemic toxicity) Warning H371: May cause damage to organs (systemic toxicity) P309+P311: IF exposed or if you feel unwell: Call a POISON CENTER or doctor/physician.
P260: Do not breathe dust/fume/gas/mist/vapours/spray.
P264: Wash ... thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P405: Store locked up.
P501: Dispose of contents/container to ...
In the acute oral toxicity study in rats (OECD TG 401), the signs such as crouching position, prostration, unsteady gait, irregular breathing, closed eyes, reduced spontaneous activity, dazed behavior or reduced reflexes were described and they were reported entirely to be non-specific symptoms (DFGMAK-Doc. 9 (1998)). Therefore, the target organ(s) were not specified, but the dose level where the symptoms occurred were considered as ones equivalent to Category 2 of the guidance values judging from LD50 value (854 mg/kg). Thus, the substance was classified as Category 2 (systemic toxicity).
9 Specific target organ toxicity - Repeated exposure Category 2 (kidney) Warning H373: May cause damage to organs through prolonged or repeated exposure (kidney) P260: Do not breathe dust/fume/gas/mist/vapours/spray.
P314: Get medical advice/attention if you feel unwell.
P501: Dispose of contents/container to ...
In the 13-week feeding study in rats (0, 200, 600, 2000, 6000 and 18000 ppm; converted values as the actual intake: 10, 30, 100, 300 and 900 mg/kg/day), significant decreases in hemoglobin and hematocrit value, significant increase in methemoglobin concentration and hyperplasia of the transitional epithelium of the urinary bladder were observed at high dose levels beyond the upper limit of the guidance values, however, no adverse effects were seen up to the dose levels of 2000 ppm. Nevertheless, degenerations and necrosis of renal tubules were observed in males at the dose levels of 600 ppm (converted value as the actual intake: 30 mg/kg, corresponding to Category 2) or higher (NTP TR 416 (1993)). In the 13-week feeding study in mice, significant decreases in hemoglobin and hematocrit value were found at the dose levels of 2000 ppm (converted value as the actual intake: 300 mg/kg/day) or higher, however, no adverse effects were noted at the dose levels below 600 ppm (converted value as the actual intake: 90 mg/kg/day) which was equivalent to the doses within the guidance values (NTP TR 416 (1993)). It was reported in another 28-day oral dose study in rats that the treatment with 200 mg/kg/day caused slight hemolytic anemia (DFGMAK-Doc.9 (1998)), but the effects were slight and were not evidenced by histopathology. Therefore, the hematological effects were not adopted for the basis of classification. While for the renal effects, degenerations and necrosis of renal tubules were recognized at the dose levels over 600 ppm (converted value as the actual intake: 30 mg/kg, corresponding to Category 2) in the 13-week feeding study in rats, therefore, the substance was classified as Category 2 (kidney).
10 Aspiration hazard Classification not possible - - - - No data available.

ENVIRONMENTAL HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
11 Hazardous to the aquatic environment (Acute) Category 3 - - H402: Harmful to aquatic life P273: Avoid release to the environment.
P501: Dispose of contents/container to ...
Classified into Category 3 from its 72h-ErC50 = 48.6 mg/L for algae (Scenedesmus subspicatus) (IUCLID, 2000).
11 Hazardous to the aquatic environment (Long-term) Category 3 - - H412: Harmful to aquatic life with long lasting effects P273: Avoid release to the environment.
P501: Dispose of contents/container to ...
Classified into Category 3 since its acute toxicity is Category 3 and it is not rapidly degradable (BOD degradation rate: 0% (Biodegradation and Bioconcentration of Existing Chemical Substances under the Chemical Substances Control Law, 1976)).
12 Hazardous to the ozone layer Classification not possible - - - - This substance is not listed in Annexes to the Montreal Protocol.


NOTE:
* Classification was conducted by relevant Japanese Ministries in accordance with GHS Classification Guidance for the Japanese Government, and is intended to provide a reference for preparing GHS labelling and SDS for users.
* This is a provisional English translation of classification results and is subject to revision without notice.
* The responsibility for any resulting GHS labelling and SDS referenced from this site is with users.

Reference:
Reference Manual

Definitions / Abbreviations

Model Label by MHLW

MHLW Website (in Japanese Only)

Model SDS by MHLW

MHLW Website (in Japanese Only)


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