GHS Classification Result

Chemical Name：O,O-Diethyl dithiophosphate
CAS：298-06-6

Result：
ID: 22A4219
Classifier: Ministry of Health, Labour and Welfare （MHLW）, Ministry of the Environment （MOE）
Year Classified: FY2010
Reference Manual: GHS Classification Guidance by the Japanese Government （July, 2010）

PHYSICAL HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
1 Explosives Not applicable - - - - There are no chemical groups associated with explosive properties present in the molecule.
2 Flammable gases （including chemically unstable gases） Not applicable - - - - Liquid (GHS definition)
3 Aerosols Not applicable - - - - Not aerosol products.
4 Oxidizing gases Not applicable - - - - Liquid (GHS definition)
5 Gases under pressure Not applicable - - - - Liquid (GHS definition)
6 Flammable liquids Category 4 - Warning H227: Combustible liquid P370+P378: In case of fire: Use ... for extinction.
P403+P235: Store in a well-ventilated place. Keep cool.
P210: Keep away from heat/sparks/open flames/hot surfaces. - No smoking.
P280: Wear protective gloves/protective clothing/eye protection/face protection.
P501: Dispose of contents/container to ... 		The flash point 82 degC (open-cup) (Ullmanns (E) (6th, 2003)) was obtained and it is estimated that the flash point measured by the closed-cup method fall within the range of > 60 degC and <= 93 degC. Therefore the substance was judged to correspond to Category 4.
7 Flammable solids Not applicable - - - - Liquid (GHS definition)
8 Self-reactive substances and mixtures Not applicable - - - - There are no chemical groups present in the molecule associated with explosive or self-reactive properties.
9 Pyrophoric liquids Not classified - - - - Since its autoignition temperature is 121 - 122 degC (15710 Chemical Products (2010)), it is considered that the substance does not ignite at room temperature.
10 Pyrophoric solids Not applicable - - - - Liquid (GHS definition)
11 Self-heating substances and mixtures Classification not possible - - - - Test methods applicable to liquid substances are not available.
12 Substances and mixtures which, in contact with water, emit flammable gases Not applicable - - - - The chemical structure of the substance does not contain metals or metalloids (B, Si, P, Ge, As, Se, Sn, Sb, Te, Bi, Po, At).
13 Oxidizing liquids Classification not possible - - - - Classification is not possible since no data are available though the substance is an organic compound containing oxygen (but not fluorine or chlorine) which is chemically bonded to the elements other than carbon or hydrogen.
14 Oxidizing solids Not applicable - - - - Liquid (GHS definition)
15 Organic peroxides Not applicable - - - - Organic compounds containing no bivalent -O-O- structure
16 Corrosive to metals Classification not possible - - - - No data available.

HEALTH HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
1 Acute toxicity （Oral） Category 4 Warning H302: Harmful if swallowed P301+P312: IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell.
P264: Wash ... thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P330: Rinse mouth.
P501: Dispose of contents/container to ... 		Based on the estimated LD50 value of 300 - 2000 mg/kg resulting from the acute oral study for female rats (OECD TG 423, GLP-compliant) ("Toxicity Testing Reports of Environmental Chemicals"(Chemicals Investigation Promoting Council) (Access on Nov. 2010)), the substance was classified as Category 4.
1 Acute toxicity （Dermal） Classification not possible - - - - No data available.
1 Acute toxicity （Inhalation: Gases） Not applicable - - - - Liquid (GHS definition)
1 Acute toxicity （Inhalation: Vapours） Classification not possible - - - - No data available.
1 Acute toxicity （Inhalation: Dusts and mists） Category 4 Warning H332: Harmful if inhaled P304+P340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P261: Avoid breathing dust/fume/gas/mist/vapours/spray.
P271: Use only outdoors or in a well-ventilated area.
P312: Call a POISON CENTER or doctor/physician if you feel unwell. 		Based on the rat LC50 value of 1.64 mg/L/4hrs (RTECS (2010), original literature:"HPV321 U.S. Environmental Protection Agency; High Production Volume (HPV) Challenge; O,O-diethyl Dithiophosphate (2003)), after reviewing the original literature, the substance was classified as Category 4. The LC50 value (1.64 mg/L) was higher than the saturated vapor pressure concentration (0.584 mg/L), the criterion values for dust or mist were adopted.
2 Skin corrosion/irritation Not classified - - - - Based on the documented case of "mild" resulted from the rabbit test in which 500 mg of the substance was applied for 24 hours (RTECS (2010), HPV321 U.S. Environmental Protection Agency; High Production Volume (HPV) Challenge; O,O-diethyl Dithiophosphate (2003)), after reviewing original literature, the substance was classified as "Not classified" in the JIS classification (Category 3 in the UN-GHS classification).
3 Serious eye damage/eye irritation Category 2A Warning H319: Causes serious eye irritation P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P337+P313: If eye irritation persists: Get medical advice/attention.
P264: Wash ... thoroughly after handling.
P280: Wear protective gloves/protective clothing/eye protection/face protection. 		Based on the documented case of "severe" resulted from the rabbit test in which 0.1 mg of the substance was instilled to rabbit eyes (RTECS (2010), HPV321 U.S. Environmental Protection Agency; High Production Volume (HPV) Challenge; O,O-diethyl Dithiophosphate (2003)), after reviewing original literature, the substance was classified as Category 2A.
4 Respiratory sensitization Classification not possible - - - - No data available.
4 Skin sensitization Classification not possible - - - - No data available.
5 Germ cell mutagenicity Classification not possible - - - - The classification was not possible due to lack of in vivo test data. As relevant information, as for in vitro studies, positive results in the Ames test (OECD TG471, GLP-compliant) and the chromosome aberration test using CHL/IU cells (OECD TG473, GLP-compliant) were reported ("Toxicity Testing Reports of Environmental Chemicals"(Chemicals Investigation Promoting Council) (Access on Nov. 2010)).
6 Carcinogenicity Classification not possible - - - - No data available.
7 Reproductive toxicity Classification not possible - - - - In the combined repeated dose toxicity study with the reproduction/developmental toxicity screening test by oral administration in rats (OECD TG 422, GLP-compliant), failure of the pregnancy by the testicular toxicity of the male animals and significantly lower viability index on day 4 of female pups were observed in 300 mg/kg group, at which severe effects on general toxicity were seen as the maternal toxicity such as salivation, loose stools and suppression of body weight gain and deaths in half of the animals during treatment period ("Toxicity Testing Reports of Environmental Chemicals"(Chemicals Investigation Promoting Council) (Access on Nov. 2010)). Therefore, this dose level was not adopted for the classification. No other effects of this substance on the reproductive index were detected, however, since fetal inspections at the end stage of pregnancy period were not carried out for evaluation of developmental effects, data were insufficient. Therefore, the substance was classified as "Classification not possible".
8 Specific target organ toxicity - Single exposure Classification not possible - - - - In the acute oral study in rats, salivation was observed immediately after administration at 300 mg/kg, and no abnormalities were found at necropsy. All three rats died by the following day at 2000 mg/kg, and clinical signs and necropsy findings observed were reported as follows: salivation, decreased locomotor activity, prone position, cyanosis, and bradypnea, and perforation of the stomach, dark reddish patch in mucous of the glandular stomach, thickening of the mucous of the glandular stomach and/or the forestomach, and ascites, respectively ("Toxicity Testing Reports of Environmental Chemicals" (Chemicals Investigation Promoting Council) (Access on Nov. 2010)). Since it was difficult to specify the target organ(s) merely from the information described above and there were no other treatment-related obvious findings at the dose levels within the guidance values, "Classification not possible" was justified. As relevant information, it was reported that the substance had a direct irritating effect on the mucosa of the gastrointestinal tract, and that these damages of the gastrointestinal tract might cause to death ("Toxicity Testing Reports of Environmental Chemicals" (Chemicals Investigation Promoting Council) (Access on Nov. 2010)). However, since the injuries of the gastrointestinal tract mentioned above were considered as the effects of local irritation, these findings were not evidenced to classify.
9 Specific target organ toxicity - Repeated exposure Category 2 (testis) Warning H373: May cause damage to organs through prolonged or repeated exposure (testis) P260: Do not breathe dust/fume/gas/mist/vapours/spray.
P314: Get medical advice/attention if you feel unwell.
P501: Dispose of contents/container to ... 		In the combined repeated dose oral toxicity study with the reproduction/developmental toxicity screening test in rats (OECD TG 422, GLP-compliant), weights of the testes and epididymides reduced at 300 mg/kg, and pathological examinations revealed the testicular dysfunction at 100 and 300 mg/kg (converted values as that of 90-day study: 46.7 - 140 mg/kg/day) ("Toxicity Testing Reports of Environmental Chemicals" (Chemicals Investigation Promoting Council) (Access on Nov. 2010)). Additionally, it was also reported that the testicular dysfunction was speculated from the histopathological findings at 300 mg/kg as alterations caused by injuries in Sertoli cells and was considered as delayed typed- or progressive lesions ("Toxicity Testing Reports of Environmental Chemicals" (Chemicals Investigation Promoting Council) (Access on Nov. 2010)). Taking it into consideration for the above information and the effects seen up to the doses within Category 2, the substance was classified as Category 2 (testis).
10 Aspiration hazard Classification not possible - - - - No data available.

ENVIRONMENTAL HAZARDS
Hazard class Classification Symbol Signal word Hazard statement Precautionary statement Rationale for the classification
11 Hazardous to the aquatic environment （Acute） Category 2 - - H401: Toxic to aquatic life P273: Avoid release to the environment.
P501: Dispose of contents/container to ... 		Classified into Category 2 from its 48h-EC50 = 4.5 mg/L for crustacea (Daphnia magna) (Test for the Ecological Effect of Chemical Substances (Ministry of the Environment), 2003).
11 Hazardous to the aquatic environment （Long-term） Category 2 - H411: Toxic to aquatic life with long lasting effects P273: Avoid release to the environment.
P391: Collect spillage.
P501: Dispose of contents/container to ... 		Classified into Category 2 since its acute toxicity is Category 2 and it is not rapidly degradable (Non-biodegradable, BOD degradation rate: 0% (Biodegradation and Bioconcentration of Existing Chemical Substances under the Chemical Substances Control Law, 1988)).
12 Hazardous to the ozone layer Classification not possible - - - - This substance is not listed in Annexes to the Montreal Protocol.


NOTE:
* Classification was conducted by relevant Japanese Ministries in accordance with GHS Classification Guidance for the Japanese Government, and is intended to provide a reference for preparing GHS labelling and SDS for users.
* This is a provisional English translation of classification results and is subject to revision without notice.
* The responsibility for any resulting GHS labelling and SDS referenced from this site is with users.

Reference：
Reference Manual
Definitions / Abbreviations
Model Label by MHLW

 MHLW Website （in Japanese Only）
Model SDS by MHLW

 MHLW Website （in Japanese Only）

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