Chemical Name：4-(4-chlorophenyl)-2-phenyl-2-[(1H-1,2,4-triazol-1-yl)methyl]butanenitrile
CAS：114369-43-6

Hazard class		Classification	Symbol	Signal word	Hazard statement	Precautionary statement	Rationale for the classification
1	Acute toxicity （Oral）	Not classified	-	-	-	-	Its rat LD50 values were documented from 3 studies (> 2000, > 2000, and > 5000 mg/kg) (JMPR (1997)), and all these values fall under the "Not classified" category.
1	Acute toxicity （Dermal）	Not classified	-	-	-	-	Based on its LD50 value of > 2000 mg/kg (JMPR (1997)), the substance was classified into "Not classified" using the JIS classification criteria (Category 5 or "Not classified" in the United Nations classification).
1	Acute toxicity （Inhalation: Gases）	Not applicable	-	-	-	-	Solid (GHS definition)
1	Acute toxicity （Inhalation: Vapours）	Classification not possible	-	-	-	-	No data available.
1	Acute toxicity （Inhalation: Dusts and mists）	Classification not possible	-	-	-	-	Based on its LC50 of > 2.1 mg/L documented (JMPR (1997)), the class cannot be specified between Category 3 and 4.
2	Skin corrosion/irritation	Not classified	-	-	-	-	Based on the results that no erythema or edema was noted in the rabbit tests (JMPR (1997)).
3	Serious eye damage/eye irritation	Not classified	-	-	-	-	Based on the results that no effects of the substance were detected in the rabbit tests (JMPR (1997)).
4	Respiratory sensitization	Classification not possible	-	-	-	-	No data available.
4	Skin sensitization	Not classified	-	-	-	-	Buehler tests using guinea pigs found that the substance showed no potential for skin sensitization (JMPR (1997)). In maximization tests using guinea pigs, the use of acetone solution resulted in a 10 % positive reaction rate (negative control: 40%), and the use of diethyl phthalate resulted in a 16% positive reaction rate (negative control: 10%), indicating that the substance has weak sensitizing potential (JMPR (1997)). In maximization tests using guinea pigs, the use of either acetone or ethanol resulted in a 0% positive reaction rate, or negative (A pesticide abstract and evaluation report (Food and Agricultural Materials Inspection Center) (2007)). Based on these three sets of results, the substance was classified into "Not classified".
5	Germ cell mutagenicity	Not classified	-	-	-	-	The classification is based on negative results found in vivo mutagenicity tests using somatic cells (micronucleus tests using mouse bone marrow cells) (JMPR (1997)). In addition negative results have been also documented in in vitro tests including Ames tests and chromosomal aberration tests (JMPR (1997)).
6	Carcinogenicity	Category 2		Warning	H351: Suspected of causing cancer	P308+P313: IF exposed or concerned: Get medical advice/attention. P201: Obtain special instructions before use. P202: Do not handle until all safety precautions have been read and understood. P281: Use personal protective equipment as required. P405: Store locked up. P501: Dispose of contents/container to ...	In 78-week mixed diet administration tests using mice, higher incidences of hepatocellular adenomas and carcinomas were detected in the highest dose groups compared with control groups. In 2-year mixed diet tests using rats, increased incidences of focal cystic thyroid hyperplasia (12/70 vs. 1/70 in controls), thyroid follicular adenoma, and carcinoma (6/70 vs. 4/70 in controls) were noted in the highest dose groups. Furthermore, in 2-year mixed diet tests using male rats, which was conducted for verification, slight thyroid follicular cell hypertrophy was noted in the highest dose groups, and the dose-response relationship for thyroid follicular adenoma was seen in 2/60, 5/60, and 9/60 rats at the doses of 0, 800, and 1600 mg/kg/day, respectively. Among these positive cases, only two cases of carcinomas were found at 0 and 1600 mg/kg/day groups (all studies cited in JMPR (1997)). Since increased hepatocellular adenomas and carcinomas in mice, and increased thyroid follicular adenoma in rats were reported, the substance was classified into Category 2.
7	Reproductive toxicity	Category 2		Warning	H361: Suspected of damaging fertility or the unborn child	P308+P313: IF exposed or concerned: Get medical advice/attention. P201: Obtain special instructions before use. P202: Do not handle until all safety precautions have been read and understood. P281: Use personal protective equipment as required. P405: Store locked up. P501: Dispose of contents/container to ...	In two-generation mixed diet administration tests using rats, effects of administration on the liver, thyroid gland, and adrenal gland were seen in parental animals, but no reproductive toxicity was detected. In forced oral administration tests conducted during the organogenetic period using pregnant rats, increased early, late, and total resorptions were seen in the highest dose groups, with a corresponding reduction in the average number of fetuses per litter and average fetal weight. In addition, in forced oral administration tests conducted during the organogenetic period using pregnant rabbits, frequent resorptions and abortions were observed in the highest dose groups (both studies cited in JMPR (1997)). Based on these reports, the substance was classified into Category 2.
8	Specific target organ toxicity - Single exposure	Classification not possible	-	-	-	-	No data available.
9	Specific target organ toxicity - Repeated exposure	Category 2 (liver)		Warning	H373: May cause damage to organs through prolonged or repeated exposure (liver)	P260: Do not breathe dust/fume/gas/mist/vapours/spray. P314: Get medical advice/attention if you feel unwell. P501: Dispose of contents/container to ...	In 3-month mixed diet administration tests using mice, hepatocellular hypertrophy and single-cell necrosis in the liver were noted in mouse groups treated within Category 2 guidance doses (33-54 mg/kg). Above these concentrations, hepatocellular hypertrophy, hepatocellular vacuolation, and single-cell or focal necrosis were noted. In 3-month mixed diet administration tests using rats, the following were observed in dose groups (16-104 mg/kg) corresponding to Category 2 guidance values: hepatocellular hypertrophy, centrilobular midzonal vacuolization, mid-zonal and periportal or perilobular vacuolation, centrilobular necrosis, and thyroid follicular epithelial hyperplasia (there is a description that thyroid gland disorder is a secondary effect of liver disorder). In 1-year mixed diet administration tests using dogs, Hepatocytic hypertrophy, and minimal-to-slight multifocal vacuolation were detected in dose groups (14-56 mg/kg) corresponding to Category 2 guidance values (all studies cited in JMPR (1997)). Based on these reports, the substance was classified into Category 2 (liver).
10	Aspiration hazard	Classification not possible	-	-	-	-	No data available.

Hazard class		Classification	Symbol	Signal word	Hazard statement	Precautionary statement	Rationale for the classification
11	Hazardous to the aquatic environment （Acute）	Category 1		Warning	H400: Very toxic to aquatic life	P273: Avoid release to the environment. P391: Collect spillage. P501: Dispose of contents/container to ...	Since its 96-hour LC50 = 0.633 mg/L for crustaceans (Mysidopsis bahia) (AQUIRE, 2008), the substance was classified into Category 1.
11	Hazardous to the aquatic environment （Long-term）	Category 1		Warning	H410: Very toxic to aquatic life with long lasting effects	P273: Avoid release to the environment. P391: Collect spillage. P501: Dispose of contents/container to ...	Since its classification for acute toxicity is Category 1, and it is not rapidly degradable (BIOWIN), the substance was classified into Category 1.