Chemical Name：Disodium 6,6'-(Biphenyl-4,4'-diylbisazo)bis(4-amino-5-hydroxy-2,7-naphthalenedisulfonate); Direct Blue 6
CAS：2602-46-2

Hazard class		Classification	Symbol	Signal word	Hazard statement	Precautionary statement	Rationale for the classification
1	Acute toxicity （Oral）	Not classified	-	-	-	-	Based on the report that rat LD50 = 8.76 g/kg (HSDB, 2003), the substance was classified into the "Not classified" category.
1	Acute toxicity （Dermal）	Not classified	-	-	-	-	Based on the report that rabbit LD50 >= 8.0 g/kg (HSDB, 2003), the substance was classified into the "Not classified" category.
1	Acute toxicity （Inhalation: Gases）	Not applicable	-	-	-	-	Solid
1	Acute toxicity （Inhalation: Vapours）	Classification not possible	-	-	-	-	No data available.
1	Acute toxicity （Inhalation: Dusts and mists）	Not classified	-	-	-	-	Since no mortality was observed in rats exposed to approx. 154 mg/L for 1 hour (HSDB, 2003), we determined that LC50 is > 154 mg/L (1 hour). The estimated LC50 for 4-hour exposure= 1 hour*(> 154 mg/L)/4 hours = > 38.5 mg/L. Consequently, using classification criteria for dust, the substance was classified into the "Not classified" category.
2	Skin corrosion/irritation	Classification not possible	-	-	-	-	No data available.
3	Serious eye damage/eye irritation	Classification not possible	-	-	-	-	No data available.
4	Respiratory sensitization	Classification not possible	-	-	-	-	No data available.
4	Skin sensitization	Classification not possible	-	-	-	-	No data available.
5	Germ cell mutagenicity	Classification not possible	-	-	-	-	In treated rats, DNA adduct formation and DNA damage were observed (RTECS, accessed in October 2007), and mouse lymphoma test gave positive results (NTP DB, accessed in October 2007). However, these positive results are considered to not act as sufficient evidence in terms of reliability. Thus, classification is not possible due to insufficient test results available.
6	Carcinogenicity	Category 1B		Danger	H350: May cause cancer	P308+P313: IF exposed or concerned: Get medical advice/attention. P201: Obtain special instructions before use. P202: Do not handle until all safety precautions have been read and understood. P281: Use personal protective equipment as required. P405: Store locked up. P501: Dispose of contents/container to ...	It is rated as "2A" by Japan Society for occupational Health (2007) and "2" by the EU (2007). Based on these ratings, the substance was classified into Category 1B.
7	Reproductive toxicity	Category 1B		Danger	H360: May damage fertility or the unborn child	P308+P313: IF exposed or concerned: Get medical advice/attention. P201: Obtain special instructions before use. P202: Do not handle until all safety precautions have been read and understood. P281: Use personal protective equipment as required. P405: Store locked up. P501: Dispose of contents/container to ...	Mice were administered this substance at dose levels of 0, 50, 75, 100, or 200 mg/kg/day (administration route not provided) on days 6 through 15 of gestation, and a Cesarean section was performed on day 18 of gestation (HSDB (2003)). Decreased weight gain was observed in parental animals administered at 100 mg/kg/day or above, and cleft palates and wavy ribs were noted in the offspring administered at 75 mg/kg/day (HSDB (2003)). In this study, since malformations were observed in the offspring at doses that did not affect parental animals, the substance was classified into Category 1B.
8	Specific target organ toxicity - Single exposure	Classification not possible	-	-	-	-	Classification not possible due to lack of data. As additional background information, in rats administered single doses of the substance through forced oral administration, congestion of the lungs was reported (HSDB, 2003). Also there are other studies indicating adverse effects of this substance. However, these studies do not explain the relationship between doses and their effects. Consequently, classification is not possible based on these studies.
9	Specific target organ toxicity - Repeated exposure	Category 2 (liver)		Warning	H373: May cause damage to organs through prolonged or repeated exposure (liver)	P260: Do not breathe dust/fume/gas/mist/vapours/spray. P314: Get medical advice/attention if you feel unwell. P501: Dispose of contents/container to ...	In a 13-week mixed diet administration test using rats, biliary hyperplasia was noted (NTP TR 108, 1978). Evaluating this report, we determined that the liver is the target organ of this substance, and this effect was seen at doses that fall under the range of Category 2 guidance values. Thus, the substance was classified into Category 2 (liver).
10	Aspiration hazard	Classification not possible	-	-	-	-	No data available.