Chemical Name：(E)-3-(dimethylamino)-1-methyl-3-oxoprop-1-enyl dimethyl phosphate
CAS：141-66-2

Hazard class		Classification	Symbol	Signal word	Hazard statement	Precautionary statement	Rationale for the classification
1	Acute toxicity （Oral）	Category 2		Danger	H300: Fatal if swallowed	P301+P310: IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician. P264: Wash ... thoroughly after handling. P270: Do not eat, drink or smoke when using this product. P321: Specific treatment （see ... on this label）. P330: Rinse mouth. P405: Store locked up. P501: Dispose of contents/container to ...	SPECIES: Rat ENDPOINT: LD50 VALUE: 16 mg/kg REFERENCE SOURCE: ACGIH (2002)
1	Acute toxicity （Dermal）	Category 1		Danger	H310: Fatal in contact with skin	P302+P350: IF ON SKIN: Gently wash with plenty of soap and water. P262: Do not get in eyes, on skin, or on clothing. P264: Wash ... thoroughly after handling. P270: Do not eat, drink or smoke when using this product. P280: Wear protective gloves/protective clothing/eye protection/face protection. P310: Immediately call a POISON CENTER or doctor/physician. P322: Specific treatment （see ... on this label）. P361: Remove/Take off immediately all contaminated clothing. P363: Wash contaminated clothing before reuse. P405: Store locked up. P501: Dispose of contents/container to ...	Rat LD50 value = 42mg/kg (ACGIH, 2002), and rabbit LD50 value = 225mg/kg (ACGIH, 2002). The lower rat LD50 value was adopted and it was set as Category 1.
1	Acute toxicity （Inhalation: Gases）	Not applicable	-	-	-	-	Liquid (GHS definition)
1	Acute toxicity （Inhalation: Vapours）	Classification not possible	-	-	-	-	No data available
1	Acute toxicity （Inhalation: Dusts and mists）	Category 2		Danger	H330: Fatal if inhaled	P304+P340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. P403+P233: Store in a well-ventilated place. Keep container tightly closed. P260: Do not breathe dust/fume/gas/mist/vapours/spray. P271: Use only outdoors or in a well-ventilated area. P284: Wear respiratory protection. P310: Immediately call a POISON CENTER or doctor/physician. P320: Specific treatment is urgent （see ... on this label）. P405: Store locked up. P501: Dispose of contents/container to ...	Calculation was done based on rat LC50 (4 hours) value: 0.09mg/L (ACGIH, 2002) and LC50 (1 hour) value: 0.61-0.91mg/L (4-hour equivalent 0.15-0.23mg/L) (ACGIH, 2002). Since the calculated values were 0.09mg/L, they were set to category 2.
2	Skin corrosion/irritation	Classification not possible	-	-	-	-	No data available
3	Serious eye damage/eye irritation	Classification not possible	-	-	-	-	No data available
4	Respiratory sensitization	Classification not possible	-	-	-	-	No data available
4	Skin sensitization	Classification not possible	-	-	-	-	No data available
5	Germ cell mutagenicity	Classification not possible	-	-	-	-	Although there is a positive result (RTECS, 2006, HSDB, 2006) by the reverse mutation test using bacteria, there is no strong positive result at other indices of the in vitro mutagenicity test. So it cannot classify.
6	Carcinogenicity	Not classified	-	-	-	-	Since it was classified into A4 (ACGIH, 2002) in ACGIH, it was considered as the outside of Category.
7	Reproductive toxicity	Not classified	-	-	-	-	It was considered as out of Category based on the description that specific reproductive toxicity was not observed at the dose in which general toxicity is observed in parental animals in the three-generation reproduction study by the oral administration using rat (ACGIH (2002), IRIS (2006)), the description although there was no description about the general toxicity of parental animals, specific reproductive toxicity was not observed in the teratogenicity test by the oral administration using rat (ACGIH (2002), IRIS (2006)), and the description that specific reproductive toxicity was not observed at the dose in which general toxicity is observed in parental animals in intraperitoneal administration test to the pregnant mice (ACGIH (2002)).
8	Specific target organ toxicity - Single exposure	Category 1 (nervous system)		Danger	H370: Causes damage to organs (nervous system)	P307+P311: IF exposed: Call a POISON CENTER or doctor/physician. P260: Do not breathe dust/fume/gas/mist/vapours/spray. P264: Wash ... thoroughly after handling. P270: Do not eat, drink or smoke when using this product. P321: Specific treatment （see ... on this label）. P405: Store locked up. P501: Dispose of contents/container to ...	It was judged that the target organ was the nervous system because of the reports indicating inhibition of cholinesterase activity in oral ingestion cases in humans (ACGIH (2002)), and of inhibition of cholinesterase activity in plasma, hemocytes and brains in laboratory animals (HSDB (2006)), so the substance was classified as Category 1 (nervous system).
9	Specific target organ toxicity - Repeated exposure	Category 1 (nervous system)		Danger	H372: Cause damage to organs through prolonged or repeated exposure (nervous system)	P260: Do not breathe dust/fume/gas/mist/vapours/spray. P264: Wash ... thoroughly after handling. P270: Do not eat, drink or smoke when using this product. P314: Get medical advice/attention if you feel unwell. P501: Dispose of contents/container to ...	It was classified into Category 1 (nerve systems) based on the description that in an oral study for two years using the dog the cholinesterase activities inhibition accompanied by the symptom which suggests the effects on the nerve systems was observed with the dose of the guidance value range of Category 1 (ACGIH (2002) and IRIS (2006)), and based on the description that the cholinesterase activities inhibition accompanied by symptom was observed in human repeated exposure examples (ACGIH (2002)).
10	Aspiration hazard	Classification not possible	-	-	-	-	No data available

Hazard class		Classification	Symbol	Signal word	Hazard statement	Precautionary statement	Rationale for the classification
11	Hazardous to the aquatic environment （Acute）	Category 1		Warning	H400: Very toxic to aquatic life	P273: Avoid release to the environment. P391: Collect spillage. P501: Dispose of contents/container to ...	It was classified into Category 1 from 48-hour EC50=12.7ppb of Crustacea (Daphnia magna) (AQUIRE, 2003).
11	Hazardous to the aquatic environment （Long-term）	Category 1		Warning	H410: Very toxic to aquatic life with long lasting effects	P273: Avoid release to the environment. P391: Collect spillage. P501: Dispose of contents/container to ...	Classified into Category 1, since acute toxicity is Category 1, supposed not rapidly degrading (BIOWIN), and bioaccumulative potential is unknown..