Chemical Name：Diisopropyl 1,3-dithiolan-2-ylidenemalonate; isoprothiolane
CAS：50512-35-1

Hazard class		Classification	Symbol	Signal word	Hazard statement	Precautionary statement	Rationale for the classification
1	Acute toxicity （Oral）	Category 4		Warning	H302: Harmful if swallowed	P301+P312: IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. P264: Wash ... thoroughly after handling. P270: Do not eat, drink or smoke when using this product. P330: Rinse mouth. P501: Dispose of contents/container to ...	It was set as Category 4 from rat oral LD50 value (male 1190mg/kg: Agricultural Chemical Registration Data, 2001).
1	Acute toxicity （Dermal）	Not classified	-	-	-	-	From rat percutaneous LD50 value (>10250mg/kg: Agricultural Chemical Registration Data, 2001), it was set as the outside of Category.
1	Acute toxicity （Inhalation: Gases）	Not applicable	-	-	-	-	Solid (GHS definition)
1	Acute toxicity （Inhalation: Vapours）	Classification not possible	-	-	-	-	No data available
1	Acute toxicity （Inhalation: Dusts and mists）	Category 4		Warning	H332: Harmful if inhaled	P304+P340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. P261: Avoid breathing dust/fume/gas/mist/vapours/spray. P271: Use only outdoors or in a well-ventilated area. P312: Call a POISON CENTER or doctor/physician if you feel unwell.	It was set as category 4 based on rat inhalation (particulate and mist) LC50 value ( >2.77mg/L: Agricultural Chemical Registration Data, 2001). In addition, although LC50 value was not specified, 4 is in the range of 1.0
2	Skin corrosion/irritation	Not classified	-	-	-	-	Since no irritant effect was seen in rabbit skin irritation study (Agricultural Chemical Registration Data, 2001), it was classified as out of Category.
3	Serious eye damage/eye irritation	Category 2B	-	Warning	H320: Causes eye irritation	P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P337+P313: If eye irritation persists: Get medical advice/attention. P264: Wash ... thoroughly after handling.	Since reversible mild irritation was seen in the rabbit eye stimulativeness examination (Agricultural Chemical Registration Data, 2001), it was set as Category 2B.
4	Respiratory sensitization	Classification not possible	-	-	-	-	No data
4	Skin sensitization	Not classified	-	-	-	-	Classified as out of category because no sensitization reactions were found in the guinea pig sensitization test (Maximization method) (Agricultural Chemical Registration Data, 2001).
5	Germ cell mutagenicity	Not classified	-	-	-	-	The substance was regarded as outside the categories because the micronucleus test in mouse bone-marrow was negative (Agricultural Chemical Registration Data, 2001). The in vitro reverse mutation test and the in vitro chromosome aberration test were also negative.
6	Carcinogenicity	Not classified	-	-	-	-	Skin keratoacanthoma increased at the 3000ppm of highest dose for males in rat carcinogenicity tests. But increase in the hyperplastic lesion was not observed, there was no difference with control group at the time of onset, it was a benign thing and not increased for females, so it was judged that it was not depended on a primary action of this substance (Agricultural Chemical Registration Data, 2001). So it was set as the outside of Category. In addition, it was concluded that there was no human carcinogenicity in the pesticide residues safety assessment committee.
7	Reproductive toxicity	Not classified	-	-	-	-	In the rat reproduction study and the rabbit teratogenicity test, there were no reproductive toxicity/teratogenicity (Agricultural Chemical Registration Data, 2001). So it was considered as on the outside of Categry.
8	Specific target organ toxicity - Single exposure	Classification not possible	-	-	-	-	Insufficient data available.
9	Specific target organ toxicity - Repeated exposure	Classification not possible	-	-	-	-	In the subacute toxicity test the mixed feed administration during three months in rat or mouse, liver weight increase was observed in both animals at 4000 ppm (more than 200mg/kg/day) (Agricultural Chemical Registration Data, 2001). Because the observed results was out of the guidance value of Category 2, it could not be classified.
10	Aspiration hazard	Classification not possible	-	-	-	-	No data available

Hazard class		Classification	Symbol	Signal word	Hazard statement	Precautionary statement	Rationale for the classification
11	Hazardous to the aquatic environment （Acute）	Category 2	-	-	H401: Toxic to aquatic life	P273: Avoid release to the environment. P501: Dispose of contents/container to ...	It was classified into Category 2 from 96-hour LC50=8100microg/L of fishes (Rainbow trout) (MOE Risk Assessment No.2, 2003).
11	Hazardous to the aquatic environment （Long-term）	Category 2		-	H411: Toxic to aquatic life with long lasting effects	P273: Avoid release to the environment. P391: Collect spillage. P501: Dispose of contents/container to ...	Classified into Category 2, since acute toxicity was Category 2 and supposed not rapidly degrading (BIOWIN), though supposed less bio-accumulative (log Kow=2.88(PHYSPROP Database, 2005)).